Product Datasheet  
AKT1(Ab-450) Antibody  
Catalog Number: 21502  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P31749
    NCBI Protein: NP_001014431.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa.448~452 (T-I-T-P-P) derived from Human AKT1.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 60kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200
  • other_names：  
  • RAC-PK-alpha; Protein kinase B;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous level of total AKT1 protein.
  • Applications：  
  • WB IHC IF
  • Background：  
  • General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

    Xing J, et al. (1998) Mol Cell Biol 18(4): 1946-55.
    Tan Y, et al.( 1996) EMBO J; 15(17): 4629-42.
    Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385.
    Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9.
    Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.



 
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