Product Datasheet  
AML1(RUNX1) Antibody  
Catalog Number: 21477  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q01196
    NCBI Protein: NP_001116079.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa.6~10(D-A-S-T-S) derived from Human AML1(RUNX1).
  • reactivity：  
  • Hu
  • appl_detail：  
  • Predicted MW: 55kd
    Western blotting: 1:500~1:1000
  • other_names：  
  • CBFA2; EVI-1; AMLCR1
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous level of total AML1(RUNX1) protein.
  • Applications：  
  • WB
  • Background：  
  • CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The a subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.

    Mao S., Frank R.C., Zhang J., Miyazaki Y., Nimer S.D.Mol. Cell. Biol. 19:3635-3644(1999)
    Pelletier N., Champagne N., Stifani S., Yang X.-J. Oncogene 21:2729-2740(2002)
    Cho J.-Y., Akbarali Y., Zerbini L.F., Gu X., Boltax J., Wang Y., Oettgen P., Zhang D.-E., Libermann T.A.J. Biol. Chem. 279:19512-19522(2004)



 
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