Product Datasheet  
CDK4 Antibody  
Catalog Number: 21437  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P11802
    NCBI Protein: NP_000066.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa.295~299(L-H-K-D-E)derived from Human CDK4.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Predicted MW: 34kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry:1:50~1:100
  • other_names：  
  • PSK-J3; CMM3; CMM3
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous levels of total CDK4 protein.
  • Applications：  
  • WB IHC
  • Background：  

  • Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle .

    Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
    Sherr, C.J. (1996) Science 274, 1672-1677.
    Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925.
    Gabrielli, B.G. et al. (1999) J Biol Chem 274, 13961-9.




 
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