Product Datasheet  
Nanog Antibody  
Catalog Number: 21423  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q9H9S0
    NCBI Protein: NP_079141.2
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa.137~141 (K-Q-V-K-T) derived from Nano g
  • reactivity：  
  • Hu Ms
  • appl_detail：  
  • Predicted MW: 42kd
    Western blotting: 1:1000
  • other_names：  
  • Homeobox transcription factor Nanog
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous level of total Nanog protein.
  • Applications：  
  • WB
  • Background：  
  • Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation

    Do HJ, et al.Biochem Biophys Res Commun. 2007 Feb 16;353(3):770-5.
    Boyer LA, et al.Cell. 2005 Sep 23;122(6):947-56.
    Freberg CT, et al. Mol Biol Cell. 2007 May;18(5):1543-53.
    Chambers I, et al. Cell. 2003 May 30;113(5):643-55.



 
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