Product Datasheet  
Keratin 8(Ab-74) Antibody  
Catalog Number: 21307  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species:  
  • Rabbit
  • Amount:  
  • 100μgμg
  • Swiss-Prot No.:  
  • Swiss-Prot: P05787
    NCBI Protein: NP_002264.1
  • Form of Antibody:  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage:  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen:  
  • Peptide sequence around aa.72~76 (L-L-S-P-L) derived from Human Keratin 8 (CK8).
  • reactivity:  
  • Hu
  • appl_detail:  
  • Predicted MW: 55kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200
  • other_names:  
  • CK 8; CK8; CYK8; Cytokeratin endo A; K8
  • Purification:  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity:  
  • The antibody detects endogenous level of total Keratin 8 protein.
  • Applications:  
  • WB IHC IF
  • Background:  
  • Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

    Nakamichi I, et al. (2002) Mol Biol Cell; 13(10): 3441-3451.
    Prochasson P, et al. (2002) Nucleic Acids Res; 30(15): 3312-3322.
    Pankov R, et al. (1994) Proc Natl Acad Sci U S A; 91(3): 873-877.
    Thorey IS, et al. (1993) Mol Cell Biol; 13(11): 6742-6751.



 
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