Product Datasheet  
MDM2(Ab-166) Antibody  
Catalog Number: 21550  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q00987
    NCBI Protein: NP_002383.2
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa.164~168 (A-T-S-E-T) derived from Human MDM2.
  • reactivity：  
  • Hu Ms Rt Monkey
  • appl_detail：  

  • Predicted MW: 90kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200

  • other_names：  
  • HDMX, hdm2
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous level of total MDM2 protein.
  • Applications：  
  • WB IHC IF
  • Background：  

  • This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues

    Haupt, Y. et al. (1997) Nature 387, 296-299.
    Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.
    Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.
    Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.




 
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