Product Datasheet  
Tyrosine Hydroxylase(Ab-31) Antibody  
Catalog Number: 21294  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P07101
    NCBI Protein: NP_954986.2
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa.29~33(V-T-S-P-R)derived from Mouse Tyrosine Hydroxylase.
  • reactivity：  
  • Ms Rt
  • appl_detail：  
  • Predicted MW: 55-60kd
    Western blotting: 1:500~1:1000
  • other_names：  
  • TYH; DYT14; DYT5b; TH;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous levels of total Tyrosine Hydroxylase protein.
  • Applications：  
  • WB
  • Background：  
  • Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms . This enzyme is required for embryonic development since TH knockout mice die before or at birth . Levels of transcription, translation and posttranslational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH . Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels.

    Kumer, S.C. and Vrana, K.E. (1996) J Neurochem 67, 443-62.
    Bodeau-P



 
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