Product Datasheet  
Zap-70(Ab-319) Antibody  
Catalog Number: 21173  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P43403
    NCBI Protein: NP _001070.2
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa.317~321 (S-P-Y-S-D) derived from Human ZAP70.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  

  • Predicted MW: 70kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • 70 kDa zeta-associated protein; SRK; Syk-related tyrosine kinase; ZA70; ZAP-70
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous level of total Zap-70 protein.
  • Applications：  
  • WB IHC
  • Background：  

  • ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions

    Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100(2): 443-448
    Ku GM, et al. (2001) EMBO J; 20(3): 457-465
    Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96(17): 9775-9780
    Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774
    Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844
    Adjali O, et al. (2005) J Clin Invest; 115(8): 2287-2295.




 
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