Product Datasheet  
cdc25C(Ab-216) Antibody  
Catalog Number: 21145  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P30307
    NCBI Protein: NP_001781.2
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa.214~218 (S-P-S-M-P) derived from Human cdc25C.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Predicted MW: 60kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200
  • other_names：  
  • CDC25M1; MPIP3;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous level of total cdc25C protein.
  • Applications：  
  • WB IHC IF
  • Background：  
  • cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

    Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3(4): 341-348.
    Ferguson AM. et al. (2005) Mol Cell Biol. 25(7): 2853-2860.
    Donzelli M. et al. (2003) EMBO Rep. 4(7): 671-677.
    Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99(4): 1990-1995.



 
© Signalway Biotechnology All Rights Reserved.