Product Datasheet  
PDK1(Ab-241) Antibody  
Catalog Number: 21005  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: O15530
    NCBI Protein: NP_002604.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around aa.239~243 (A-N-S-F-V) derived from Human PDK1.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 63kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200
  • other_names：  
  • PDPK1, PkB kinase, Protein kinase B kinase, hPDK1, kinase PDK1
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous level of total PDK1 protein.
  • Applications：  
  • WB IHC IF
  • Background：  
  • Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development

    Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63
    Chen H, et al. (2001) Biochemistry; 40(39): 11851-9
    Sato S,et al. (2002) J Biol Chem; 277(42): 39360-7
    Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11
    Beausoleil SA, et al. (2004)Proc Natl Acad Sci U S A; 101(33): 12130-5



 
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