Product Datasheet  
PKC zeta (Phospho-Thr560) Antibody  
Catalog Number: 11958  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: Q05513;
    NCBI Gene#: 5590;
    NCBI Protein#: NP_001028753.1
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phospho rylation site of threonine560(Q-L-T(p)-P-D) derived from Human PKC zeta .
  • reactivity：  
  • Hu Ms
  • appl_detail：  
  • Western blotting: 1:500~1:1000
  • other_names：  
  • KPCZ; PKC-zeta; PKC2; PRKCZ; nPKC-zeta
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of PKC zeta only when phosphorylated at threonine 560.
  • Applications：  
  • WB
  • Background：  

  • This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

    Nakayama M, et al. (2013)Nat Cell Biol 15, 249-60. Mayanglambam A, Bhavanasi D, Vijayan KV, Kunapuli SP (2011)Biochem Pharmacol 82, 505-13. Dettori R, et al. (2009)J Biol Chem 284, 30318-27.




 
© Signalway Biotechnology All Rights Reserved.