Product Datasheet  
NF-κB p100 (Phospho-Ser872) Antibody  
Catalog Number: 11919  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: Q00653;
    NCBI Gene#: 4791;
    NCBI Protein#: NP_001070962.1
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine872(S-Q-S(p)-V-E) derived from Human NF-κB p100 .
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Western blotting: 1:500~1:1000
  • other_names：  
  • DNA-binding factor KBF2; Lymphocyte translocation chromosome 10; Lyt10; Nuclear factor NF-kappa-B p100 subunit; Oncogene Lyt-10
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of NF-κB p100 only when phosphorylated at serine 872.
  • Applications：  
  • WB
  • Background：  

  • NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity.

    Qu Z, Qing G, Rabson A, Xiao G (2004) J Biol Chem 279, 44563-72 . Xiao G, Fong A, Sun SC (2004) J Biol Chem 279, 30099-105 .




 
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