Product Datasheet  
Smad3 (Phospho-Ser213) Antibody  
Catalog Number: 11917  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P84022;
    NCBI Gene#: 4088;
    NCBI Protein#: NP_001138574.1
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine213(P-M-S(p)-P-A) derived from Human Smad3 .
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Western blotting: 1:500~1:1000
  • other_names：  
  • JV15-2; MAD-3; MADH3; Smad 3; Mothers against decapentaplegic homolog 3
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of Smad3 only when phosphorylated at serine 213.
  • Applications：  
  • WB
  • Background：  

  • Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

    Zelivianski S, Cooley A, Kall R, Jeruss JS (2010) Mol Cancer Res 8, 1375-87. Alarc¨®n C, et al. (2009)Cell 139, 757-69. Gao S, et al. (2009)Mol Cell 36, 457-68.




 
© Signalway Biotechnology All Rights Reserved.