Product Datasheet  
EPHA4 (Phospho-Tyr596) Antibody  
Catalog Number: 11834  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P54764;
    NCBI Gene#: 2043;
    NCBI Protein#: NP_004429.1.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 596 (R-T-Y(p)-V-D) derived from Human EPHA4 .
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000

  • other_names：  
  • EK8; hEK8; EPH-like kinase 8;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of EPHA4 only when phosphorylated at tyrosine 596
  • Applications：  
  • WB
  • Background：  

  • Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity.

    Rikova K., Cell 131:1190-1203(2007) [PubMed: 18083107].
    Richter M., J. Neurosci. 27:14205-14215(2007) [PubMed: 18094260].
    Fu W.Y., Nat. Neurosci. 10:67-76(2007) [PubMed: 17143272].




 
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