Product Datasheet  
ATF-4 (Phospho-Ser219) Antibody  
Catalog Number: 11831  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P18848;
    NCBI Gene#: 468;
    NCBI Protein#: NP_001666.2.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Serine 219 (N-D-S(p)-G-T) derived from Human ATF-4.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000

  • other_names：  
  • ATF4; CREB-2; DNA-binding protein TAXREB67; TXREB;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The Antibody detects endogenous levels of ATF-4 only when phosphorylated at Serine 219.
  • Applications：  
  • WB
  • Background：  

  • ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

    Sato T., Shimotohno K. J. Virol. 65:1420-1426(1991)
    Leiden J.M. Proc. Natl. Acad. Sci. U.S.A. 89:4820-4824(1992)
    Bridgeman A.M. Wright H. Nature 402:489-495(1999)




 
© Signalway Biotechnology All Rights Reserved.