Product Datasheet  
HCK (Phospho-Tyr522) Antibody  
Catalog Number: 11822  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P08631;
    NCBI Gene#: 3055;
    NCBI Protein#: NP_002101.2.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 522(S-Q-Y(p)-Q-Q) derived from Human HCK .
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000

  • other_names：  
  • p59-HCK/p60-HCK; Hemopoietic cell kinas; Tyrosine-protein kinase HCK;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of HCK only when phosphorylated at tyrosine 522.
  • Applications：  
  • WB
  • Background：  

  • The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.

    Quintrell N., Mol. Cell. Biol. 7:2267-2275(1987) [PubMed: 3496523].
    Ziegler S.F.,Mol. Cell. Biol. 7:2276-2285(1987) [PubMed: 3453117].
    Ota T., Nat. Genet. 36:40-45(2004) [PubMed: 14702039].




 
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