Product Datasheet  
E2A (Phospho-Thr355) Antibody  
Catalog Number: 11798  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P15923;
    NCBI Gene#: 6929;
    NCBI Protein#: NP_003191.1.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 355 (P-S-T(p)-P-V) derived from Human E2A .
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  

  • Immunohistochemistry: 1:50~1:100

  • other_names：  
  • E12; Tran; TCF3; TFE2; ITF1
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of E2A only when phosphorylated at threonine 355.
  • Applications：  
  • IHC
  • Background：  

  • E2A is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described.

    Kamps M.P., Cell 60:547-555(1990).
    Nourse J., Cell 60:535-545(1990).
    Grimwood J., Nature 428:529-535(2004).




 
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