Product Datasheet  
CAD (Phospho-Thr456) Antibody  
Catalog Number: 11789  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P27708;
    NCBI Gene#: 790;
    NCBI Protein#: NP_004332.2.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 456 (P-I-T(p)-P-H) derived from Human CAD.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Immunohistochemistry: 1:50~1:100

  • other_names：  
  • PYR1; CAD protein; EC 2.1.3.2; EC 3.5.2.3;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of CAD only when phosphorylated at threonine 456.
  • Applications：  
  • IHC
  • Background：  

  • The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.

    Iwahana H., Biochem. Biophys. Res. Commun. 219:249-255(1996).
    Davidson J.N., DNA Cell Biol. 9:667-676(1990).
    Olsen J.V., Cell 127:635-648(2006).




 
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