Product Datasheet  
AML1 (Phospho-Ser435) Antibody  
Catalog Number: 11783  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: Q01196;
    NCBI Gene#: 861;
    NCBI Protein#: NP_001001890.1.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Serine 435( S-N-S(p)-P-T) derived from Human AML1.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000

  • other_names：  
  • Acute myeloid leukemia 1 protein; CBFA2; CBFA2; alpha 2 subunit; RUNX1
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of AML1 only when phosphorylated at serine 435.
  • Applications：  
  • WB
  • Background：  

  • CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.

    Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).
    Sacchi N., Genes Chromosomes Cancer 11:226-236(1994).
    Nucifora G., Blood 81:2728-2734(1993).




 
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