Product Datasheet  
ITCH (Phospho-Tyr420) Antibody  
Catalog Number: 11774  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: Q96J02;
    NCBI Gene#: 83737;
    NCBI Protein#: NP_001244066.1.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 420(F-I-Y(p)-G-N) derived from Human ITCH .
  • reactivity：  
  • Hu Ms
  • appl_detail：  

  • Immunohistochemistry: 1:50~1:100

  • other_names：  
  • AIF4; AIP4; NAPP1;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of ITCH only when phosphorylated at tyrosine 420.
  • Applications：  
  • IHC
  • Background：  

  • Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain.

    Chen X., Genomics 73:238-241(2001).
    Deloukas P., Nature 414:865-871(2001).
    Wood J.D., Mol. Cell. Neurosci. 11:149-160(1998).




 
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