Product Datasheet  
TOP2A (Phospho-Thr1343) Antibody  
Catalog Number: 11763  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P11388;
    NCBI Gene#: 7153;
    NCBI Protein#: NP_001058.2.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine1343(E-K-T(p)-D-D) derived from Human TOP2A.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Western blotting: 1:500~1:1000

  • other_names：  
  • TP2A; TOP2; TOP2A; P11388-1;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of TOP2A only when phosphorylated at threonine 1343.
  • Applications：  
  • WB
  • Background：  

  • This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event.

    Tsai-Pflugfelder M., Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988).
    Wasserman R.A., Cancer Res. 53:3591-3596(1993).
    Lang A.J., Gene 221:255-266(1998).




 
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