Product Datasheet  
p53 (Phospho-Thr387) Antibody  
Catalog Number: 11718  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: P04637;
    NCBI Gene#: 7157;
    NCBI Protein#: NP_000537.3.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 387(F-K-T(p)-E-G) derived from Human p53.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Immunohistochemistry: 1:50~1:100

  • other_names：  
  • TP53; tumor protein p53; Tumor suppressor p53;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of p53 only when phosphorylated at threonine 387.
  • Applications：  
  • IHC
  • Background：  

  • Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

    Zakut-Houri R., EMBO J. 4:1251-1255(1985).
    Lamb P., Mol. Cell. Biol. 6:1379-1385(1986).
    Harlow E., Mol. Cell. Biol. 5:1601-1610(1985).




 
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