Product Datasheet  
SNAI1 (Phospho-Ser246) Antibody  
Catalog Number: 11709  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: O95863;
    NCBI Gene#: 6615;
    NCBI Protein#: NP_005976.2.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Serine 246(T-F-S(p)-R-M) derived from Human SNAI1.
  • reactivity：  
  • Hu Ms
  • appl_detail：  

  • Western blotting: 1:500~1:1000
    Immunofluorescence: 1:100~1:200

  • other_names：  
  • SNAH; SNAI; Sna; Snail;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of SNAI1 only when phosphorylated at serine 246.
  • Applications：  
  • WB IF
  • Background：  

  • Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.

    Okubo T., Cancer Res. 61:1338-1346(2001).
    Twigg S.R., Hum. Genet. 105:320-326(1999).
    Paznekas W.A., Genomics 62:42-49(1999).




 
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