Product Datasheet  
MDM2 (Phospho-Ser186/Ser188) Antibody  
Catalog Number: 11702  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: Q00987;
    NCBI Gene#: 4193;
    NCBI Protein#: NP_001138811.1.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Ser186/Ser188(H-K-S(p)-D-S(p)-I-S) derived from Human MDM2.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Immunofluorescence: 1:100~1:200

  • other_names：  
  • Oncoprotein Mdm2; p53-binding protein Mdm2; Ubiquitin-protein ligase E3 Mdm2;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of MDM2 only when phosphorylated at serine 186 and serine 188.
  • Applications：  
  • IF
  • Background：  

  • This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues

    M Zhou, Blood, Mar 1995; 85: 1608 - 1614.
    Susan M. Mendrysa, Mol. Cell. Biol., Mar 2000; 20: 2023 - 2030.
    M. Saeed Sheikh, Cancer Res., Jul 1993; 53: 3226 - 3228.




 
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