Product Datasheet  
BAD (Phospho-Ser91/128) Antibody  
Catalog Number: 11685  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#: Q92934;
    NCBI Gene#: 572;
    NCBI Protein#: NP_004313.1.
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C/1 year
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Serine 91 (E-P-S(p)-P-F) derived from Human BAD or Serine 128 (E-P-S(p)-P-F) derived from Mouse BAD.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  

  • Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • BAD; BBC6; BCL2L8; Bcl- XL/Bcl-2 associated death promoter;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous levels of BAD only when phosphorylated at serine 91/ serine 128.
  • Applications：  
  • WB IHC
  • Background：  

  • The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

    Wang H.-G., Cell 87:629-638(1996).
    Ottilie S., J. Biol. Chem. 272:30866-30872(1997).
    The MGC Project Team, Genome Res. 14:2121-2127(2004).




 
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