Product Datasheet  
PDHK1(Phospho-Tyr243) Antibody  
Catalog Number: 11597  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q15118
    NCBI Protein: NP_001265478.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 243(D-L-Y(p)-Y-I) derived from Human PDHK1.
  • reactivity：  
  • Hu Rt
  • appl_detail：  

  • Western blotting: 1:500~1:1000 

  • other_names：  
  • PDK1; PDH kinase 1
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of PDHK1 only when phosphorylated at tyrosine 243.
  • Applications：  
  • WB
  • Background：  
  •  Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
     
    Gudi R., Bowker-Kinley M.M., Kedishvili N.Y., Zhao Y., Popov K.M.J. Biol. Chem. 270:28989-28994(1995)
    The MGC Project TeamGenome Res. 14:2121-2127(2004)

     




 
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