Product Datasheet  
MEK5(phospho-Ser311/ Thr315) Antibody  
Catalog Number: 11559  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q62862
    NCBI Protein: NP_001029159.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Serine 311 and threonine 315 (V-N-S(p)I-A-K-T(p)-Y-V) derived from Rat MEK5.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 46kd
    Western blotting: 1:500~1:1000
  • other_names：  
  • Map2k5
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of MEK5 only when phosphorylated at serine 311 or threonine 315.
  • Applications：  
  • WB
  • Background：  
  • Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.

    English J.M., Vanderbilt C.A., Xu S., Marcus S., Cobb M.H.J. Biol. Chem. 270:28897-28902(1995)



 
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