Product Datasheet  
Cdc25B(Phospho-Ser149) Antibody  
Catalog Number: 11553  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot:P30306
    Gene ID:12531
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Serine 149 (F-R-S(p)-L-P) derived from Mouse Cdc25B.
  • reactivity：  
  • Ms
  • appl_detail：  

  • Predicted MW: 62kd
    Western blotting: 1:500~1:1000

  • other_names：  
  • CDC25B
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149.
  • Applications：  
  • WB
  • Background：  

  • Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

    Li G, et al. (2010) Retrovirology.7:59.
    Wang Z,et al. (2010) BMC Cancer.10:233.
    Lucci MA,et al. (2010) Cell Oncol.32(5-6):361-72.




 
© Signalway Biotechnology All Rights Reserved.