Product Datasheet  
Cyclin E1(phospho-Thr395) Antibody  
Catalog Number: 11541  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P24864
    NCBI Protein: NP_001229.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 395 (L-L-T(p)-P-P)derived from Human Cyclin E1
  • reactivity：  
  • Hu
  • appl_detail：  
  • Predicted MW: 48kd
    Western blotting: 1:1000
  • other_names：  
  • CCNE; CCNE1;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of Cyclin E1 only when phosphorylated at threonine 395.
  • Applications：  
  • WB
  • Background：  
  • The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition.

    Won K.A., Reed S.I.EMBO J. 15:4182-4193(1996)
    Welcker M., Singer J., Loeb K.R., Grim J., Bloecher A., Mol. Cell 12:381-392(2003)



 
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