Product Datasheet  
Aurora A(phospho-Thr288) Antibody  
Catalog Number: 11519  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: O14965
    NCBI Protein: NP_003591.2
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of threonine 288 (R-T-T(p)-L-M) derived from Human Aurora A.
  • reactivity：  
  • Hu Ms
  • appl_detail：  

  • Predicted MW: 48kd
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • AIK; ARK1; AURA; BTAK; STK6
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of Aurora A only when phosphorylated at threonine 288.
  • Applications：  
  • WB IHC
  • Background：  

  • Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity By similarity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.

    Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
    Hauf, S. et al. (2003) J Cell Biol 161, 281-94.
    Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
    Zhou, H. et al. (1998) Nat Genet 20, 189-93.




 
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