Product Datasheet  
eIF4B(phospho-Ser422) Antibody  
Catalog Number: 11513  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P23588
    NCBI Protein: NP_001408.2
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of Serine 422 (T-G-S(p)-E-S) derived from Human eIF4B.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 80kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of eIF4B only when phosphorylated at Serine 422.
  • Applications：  
  • WB IHC
  • Background：  
  • Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

    Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.
    Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260, 5493-5497.
    Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109, 1467-1481.



 
© Signalway Biotechnology All Rights Reserved.