Product Datasheet  
PI3 Kinase p85/p55 (phospho-Tyr467/199)Antibody  
Catalog Number: 11508  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot:P27986
    Gene ID:5295
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 467 (L-Y(p)-E-E-Y) derived from Human PI3 Kinase p85/p55.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  

  • Predicted MW: 55, 85kd
    Western blotting: 1:500~1:1000

    IF 1:100-1:500

  • other_names：  
  • p85, AGM7, p85-ALPHA, p55, p55-GAMMA
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of total PI3 Kinase p85/p55 only when phosphorylated at tyrosine 467/199.
  • Applications：  
  • WB,IF
  • Background：  

  • Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1./Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.

    Stoyanov, B. et al. (1995) Science 269, 690-3.
    Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
    Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.




 
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