Product Datasheet  
Ephrin-B2(Phospho-Tyr330) Antibody  
Catalog Number: 11189  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P52799
    NCBI Protein: NP_004084.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 330 (N-I-Y(p)-Y-K) derived from Human Ephrin-B2
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 37kd
    Western blotting: 1:500~1:1000
  • other_names：  
  • HTKL; EPLG5; Htk-L; LERK5; MGC126226
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Specificity：  
  • The antibody detects endogenous levels of Ephrin-B2 only when phosphorylated at tyrosine 330.
  • Applications：  
  • WB
  • Background：  
  • Adducins are a family of cytoskeleton proteins encoded by three genes (a, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. a- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. a- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.

    Chrencik JE,et al.( 2006) J Biol Chem;281(38):28185-28192.
    Kertesz N, et al. (2006) Blood; 107(6):2330-2338.
    Noren NK, et al. (2004) Proc Natl Acad Sci USA; 101(15):5583-5588.



 
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