Product Datasheet  
Smad3(Phospho-Ser425) Antibody  
Catalog Number: 11325  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P84022
    NCBI Protein: NP_001138574.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine 425 (C-S-S-V-S(p)) derived from Human Smad3.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 52kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
  • Specificity：  
  • The antibody detects endogenous level of Smad3 only when phosphorylated at serine 425.
  • Applications：  
  • WB IHC
  • Background：  
  • Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

    Shi W, et al. J Cell Sci. 2007 Apr 1;120(Pt 7):1216-24
    Seong HA, et al. J Biol Chem. 2007 Apr 20;282(16):12272-89
    Wordinger RJ, et al. Invest Ophthalmol Vis Sci. 2007 Mar;48(3):1191-200
    LeClair RJ, et al. Circ Res. 2007 Mar 30;100(6):826-33



 
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