Product Datasheet
Mouse Anti-Human CD40L (CD154)
Catalog Number:
Technical:tech@swbio.com
Information:info@swbio.com
Description
- Form of Antibody:
- Lyophilized from a 0.2μm filtered solution in phosphate buffered saline (PBS) and reconstitute with sterile PBS .
- Storage:
- Store protected from light at 2-8˚C. Do not freeze. The expiration date is indicated on the vial label.
- Immunogen:
- L929/CD40L transfected cells
- appl_detail:
- Blockade of Receptor-ligand Interaction: In a functional ELISA, 1-5 ?g/mL of this antibody will block 50% of the binding of 100 ng/mL of biotinylated Recombinant Human CD40L to immobilized Recombinant Human CD40 Fc Chimera coated at 1 ?g/mL (100 ?L/well).
Preparation:This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with L929/CD40L transfected cells). The monoclonal antibody was purified from tissue culture supernatant or ascites by protein G affinity chromatography.
Product Notices:This reagent has been pre-diluted for use at the recommended Volume per Test.
We typically use 1 10^6 cells in a 100-?l experimental sample (per test).
An isotype control should be used at the same concentration as the antibody of interest.
- other_names:
- CD40L , CD154 , TNFSF5
- Specificity:
- This antibody recognizes human CD40L in FACS.
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