Product Datasheet  
p150 CAF1 Antibody  
Catalog Number: 49950  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#:Q13111
  • Form of Antibody：  
  • 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Recombinant protein within human p150 CAF1 aa 100-300.
  • reactivity：  
  • Hu
  • appl_detail：  
  • WB: 1:500-1:2,000
    IHC: 1:50-1:200
    ICC/IF: 1:50-1:200
    IP: 1:10-1:50
    FC: 1:50-1:100
  • other_names：  
  • CAF 1 antibody
    CAF 1 subunit A antibody
    CAF antibody
    CAF I 150 kDa subunit antibody
    CAF I p150 antibody
    CAF-1 subunit A antibody
    CAF-I 150 kDa subunit antibody
    CAF-I p150 antibody
    CAF1 antibody
  • Purification：  
  • ProA affinity purified
  • Specificity：  
  • Applications：  
  • WB,ICC,IF,IP,IHC,FC
  • Background：  
  • Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated Histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.



 
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