Product Datasheet  
4E-BP1/2/3 (Phospho-Thr 45) Antibody  
Catalog Number: 13321  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Mouse
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#:O95477
  • Form of Antibody：  
  • 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • peptide
  • reactivity：  
  • Hu, Ms, Rt
  • appl_detail：  
  • WB: 1:100-1:1,000


    IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  • other_names：  
  • 4E-BP1 antibody
    4EBP1 antibody
    4EBP1_HUMAN antibody
    BP 1 antibody
    eIF4E binding protein 1 antibody
    eIF4E-binding protein 1 antibody
    Eif4ebp1 antibody
    Eukaryotic translation initiation factor 4E-binding protein 1 antibody
    PHAS-I antibody
    PHASI antibody
    Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
  • Purification：  
  • ProA affinity purified
  • Specificity：  
  • Applications：  
  • WB, IP, IF
  • Background：  
  • The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). 4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyper-phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3-kinase/Akt pathway and the FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues and phosphorylation by FRAP/mTOR on Threonine 37 and Threonine 46 of human 4E-BP1 may prime it for sub-sequent phosphorylation at sites including Serine 65 and Threonine 70. The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 and Threonine 69. In vitro, 4E-BP1 is also phosphorylated by ataxia telangiectasia (ATM) at human Serine 112 (rat Serine 111) in response to an increase in insulin levels.



 
© Signalway Biotechnology All Rights Reserved.