Product Datasheet  
β-actin antibody  
Catalog Number: 39199  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species:  
  • Mouse
  • Amount:  
  • 100μgμg
  • Swiss-Prot No.:  
  • Swiss-Prot#: Q1KLZ0
    NCBI Gene ID: 60
  • Form of Antibody:  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage:  
  • Store at -20˚C
  • Immunogen:  
  • Fusion protein of human β-actin.
  • reactivity:  
  • Hu Ms Rt
  • appl_detail:  
  • Western blotting: 1:2000 - 1:5000
  • other_names:  
  • ACTB;PS1TP5BP1;beta-actin; β-actin
  • Purification:  
  • Antibodies were purified by affinity purification using immunogen.
  • Specificity:  
  • The antibody detects endogenous level of total β-actin antibody.
  • Applications:  
  • WB
  • Background:  
  • Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha ( ACTC1, cardiac muscle 1), alpha 1 ( ACTA1, skeletal muscle) and 2 ( ACTA2, aortic smooth muscle), beta ( ACTB), gamma 1 ( ACTG1) and 2 ( ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta Actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody was generated by full length beta actin protein and can cross-react with other actins.



 
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