Product Datasheet  
p56Dok-2(Phospho-Tyr299) Antibody  
Catalog Number: 11278  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: O60496
    NCBI Protein: NP_003965.2
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 299 (G-E-Y(p)-A-V) derived from Human p56Dok-2.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Predicted MW: 56kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200

  • other_names：  
  • DOK2
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of p56Dok-2 only when phosphorylated at tyrosine 299.
  • Applications：  
  • WB IHC IF
  • Background：  

  • DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation

    Feng Cong, et,al. (1999) Mol. Cell. Biol ; 19: 8314 - 8325.
    Serge Lemay, et,al. (2000) Mol. Cell. Biol ; 20: 2743 - 2754.
    Ute Schaeper, et,al.(2000) J. Cell Biol ; 149: 1419.
    Miyuki Honma, et,al. (2006) Genes Cells; 11: 143 - 151.




 
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