Product Datasheet  
p62Dok(phospho-Tyr398) Antibody  
Catalog Number: 11277  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q99704
    NCBI Protein: NP_001372.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 398 (E-G-Y(p)-E-L) derived from Human p62Dok.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Predicted MW: 62kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • DOK1
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of p62Dok only when phosphorylated at tyrosine 398.
  • Applications：  
  • WB IHC
  • Background：  
  • DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.

    Michael J. Wick, et al. (2001) J. Biol. Chem ; 276: 42843 - 42850.
    Paul D. Simoncic, et al. (2006) Mol. Cell. Biol ; 26: 4149 - 4160.
    Nadia Dub



 
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