Product Datasheet  
Cortactin(Phospho-Tyr466) Antibody  
Catalog Number: 11272  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q14247
    NCBI Protein: NP_005222.2
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 466 (P-V-Y(p)-E-T) derived from Human CORTACTIN.
  • reactivity：  
  • Hu Ms
  • appl_detail：  

  • Predicted MW: 85kd
    Western blotting: 1:500~1:1000

  • other_names：  
  • Amplaxin; CTTN; EMS1; SRC8;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of Cortactin only when phosphorylated at tyrosine 466.
  • Applications：  
  • WB
  • Background：  

  • Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

    Luo C, et al. (2006) J Biol Chem ; 281(40):30081-30093
    Head JA, et al. (2003) Mol Biol Cell ;14(8):3216-3229
    Li Y, et al. (2000) J Biol Chem ; 275(47): 37187-37193
    Kinley AW, et al. (2003) Curr Biol ; 13(5): 384-393




 
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