Product Datasheet  
p70 S6 Kinase(Phospho-Ser411) Antibody  
Catalog Number: 11269  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P23443
    NCBI Protein: NP_003152.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine 411 (I-R-S(p)-P-R) derived from Human p70 S6 Kinase.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 70 85 kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200
  • other_names：  
  • KS6B1; P70-S6K; RPS6KB1; S6K;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at serine 411.
  • Applications：  
  • WB IHC IF
  • Background：  
  • Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.

    Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851
    Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84.
    Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c
    Le, X.F, et al. (2003) Oncogene 22: 484



 
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