Product Datasheet  
c-Kit(Phospho-Tyr721) Antibody  
Catalog Number: 11240  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P10721
    NCBI Protein: NP_000213.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 721 (N-E-Y(p)-M-D) derived from Human c-Kit.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Predicted MW: 145kd
    Western blotting: 1:500~1:1000
  • other_names：  
  • KIT , CD117, SCFR, PBT
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of c-Kit only when phosphorylated at tyrosine 721.
  • Applications：  
  • WB
  • Background：  
  • This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)

    Nocka, K. et al. (1990) EMBO J. 9, 1805-1813.
    Hirota, S. et al. (1998) Science 279, 577-580.
    Blume-Jensen, P. et al. (2000) Nat. Genet. 24, 157-162.
    Sattler, M. et al. (1997) J. Biol. Chem. 272, 10248-10253.
    Gommerman, J.L. et al. (1997) J. Biol. Chem. 272, 30519-30525.



 
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