Product Datasheet  
VASP(Phospho-Ser157) Antibody  
Catalog Number: 11214  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P50552
    NCBI Protein: NP_003361.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine 157 (R-V-S(p)-N-A) derived from Human VASP.
  • reactivity：  
  • Hu Rt
  • appl_detail：  
  • Predicted MW: 50kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of VASP only when phosphorylated at serine 157.
  • Applications：  
  • WB IHC
  • Background：  
  • Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

    Zhao WM, et al. (2001) EMBO J 20(9): 2315-2325.
    Millard TH, et al. (2005) EMBO J 24(2): 240-250.
    K



 
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