Product Datasheet  
Paxillin(Phospho-Tyr31) Antibody  
Catalog Number: 11201  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P49023
    NCBI Protein: NP_001074324.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 31 (T-P-Y(p)-S-Y) derived from Human Paxillin.
  • reactivity：  
  • Hu
  • appl_detail：  

  • Predicted MW: 68kd
    Western blotting: 1:500~1:1000

  • other_names：  
  • PAXI; PXN;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 31.
  • Applications：  
  • WB
  • Background：  

  • An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions

    Davidson D, et al. (2001) EMBO J 20(13): 3414-3426.
    Fleming I, et al. (1999) Proc Natl Acad Sci U S A 96(3): 1123-1128.
    Goldberg MB. (2001) Microbiol Mol Biol Rev 65(4): 595-626.
    Kook S, et al. (2000) Mol Biol Cell 11(3): 929-939.




 
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