Product Datasheet  
cdc25A(Phospho-Ser76) Antibody  
Catalog Number: 11138  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P30304
    NCBI Protein: NP_001780.2
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine 76 (M-G-S(p)-S-E) derived from Human cdc25A.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Predicted MW: 70kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
  • other_names：  
  • CDC25M3; MPIP1;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of cdc25A only when phosphorylated at serine 76.
  • Applications：  
  • WB IHC
  • Background：  
  • Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

    Jin J, et al. (2003) Genes 17(24): 3062-74.
    Shimuta K, et al. (2002) EMBO J 21(14): 3694-703.



 
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