Product Datasheet  
G3BP-1(Phospho-Ser232) Antibody  
Catalog Number: 11082  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q13283
    NCBI Protein: NP_005745.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of serine 232 (S-S-S(p)-P-A) derived from Human G3BP-1.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Predicted MW: 60kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100
    Immunofluorescence: 1:100~1:200
  • other_names：  
  • G3BP; GAP SH3-domain binding protein 1;
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of G3BP-1 only when phosphorylated at serine 232.
  • Applications：  
  • WB IHC IF
  • Background：  
  • May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.

    Tao WA, et al. (2005) Nat Methods. 2(8): 591-598.
    Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.
    Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A.101 (33): 12130-12135.
    Tourrihre H, et al. (2003) J Cell Biol.160 (6): 823-831.



 
© Signalway Biotechnology All Rights Reserved.