Product Datasheet  
STAT1(Phospho-Tyr701) Antibody  
Catalog Number: 11044  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: P42224
    NCBI Protein: NP _009330.1
  • Form of Antibody：  
  • Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
  • Immunogen：  
  • Peptide sequence around phosphorylation site of tyrosine 701 (T-G-Y(p)-I-K) derived from Human STAT1.
  • reactivity：  
  • Hu Ms
  • appl_detail：  

  • Predicted MW: 84,91kd
    Western blotting: 1:500~1:1000
    Immunohistochemistry: 1:50~1:100

  • other_names：  
  • CANDF7, ISGF-3, STAT91
  • Purification：  
  • Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Specificity：  
  • The antibody detects endogenous level of STAT1 only when phosphorylated at tyrosine 701.
  • Applications：  
  • WB IHC
  • Background：  

  • Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

    Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120.
    Durbin J E, et al. (1996) Cell. 84: 443-450.
    Meraz M A, et al. (1996) Cell. 84: 431-442.
    Wakao H, et al. (1994) EMBO J. 13: 2182-2191.
    Demoulin J, B. et al. (1999) J Biol Chem. 274: 25855-258
    Ihle J N, et al. (1994) Trends Biochem Sci. 19: 222-227.




 
© Signalway Biotechnology All Rights Reserved.