Product Datasheet  
DNA ligase IV Antibody  
Catalog Number: 35335  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#:P49917;
    NCBI Gene#:3981
  • Form of Antibody：  
  • Rabbit IgG in 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Recombinant fragment corresponding to a region within amino acids 207 and 611 of DNA ligase IV.
  • reactivity：  
  • Hu
  • appl_detail：  
  • Western blotting: 1:500-1:3000
  • other_names：  
  • LIG4 antibody; DNA ligase 4 antibody; polynucleotide ligase antibody; sealase antibody; DNA repair enzyme antibody; DNA ligase IV antibody; polydeoxyribonucleotide synthase [ATP] 4 antibody; DNA joinase antibody; "ligase IV; DNA; ATP-dependent antibody"
  • Purification：  
  • Antibodies were purified by antigen-affinity chromatography.
  • Specificity：  
  • The antibody detects endogenous levels of total DNA ligase IV protein.
  • Applications：  
  • WB
  • Background：  
  • The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]



 
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