Product Datasheet  
MED8 Antibody  
Catalog Number: 34778  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot: Q96G25
    NCBI Gene ID: 112950
  • Form of Antibody：  
  • Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Synthesized peptide derived from Internal of human MED8.
  • reactivity：  
  • Hu Ms Rt
  • appl_detail：  
  • Western blotting: 1:500~1:3000

  • other_names：  
  • Activator-recruited cofactor 32 kDa component; ARC32; MED8; Mediator complex subunit 8; Mediator of RNA polymerase II transcription subunit 8
  • Purification：  
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Specificity：  
  • The antibody detects endogenous levels of total MED8 protein.
  • Applications：  
  • WB
  • Background：  
  • Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins.

    Brower C.S., Proc. Natl. Acad. Sci. U.S.A. 99:10353-10358(2002).
    Gregory S.G., Nature 441:315-321(2006).
    Naeaer A.M., Nature 398:828-832(1999).



 
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